biotin sp goat anti syrian hamster igg h l  (Vector Laboratories)

Journal: eLife

Article Title: Immunogenicity and safety of a live-attenuated SARS-CoV-2 vaccine candidate based on multiple attenuation mechanisms

doi: 10.7554/elife.97532

Figure Lengend Snippet: Figure 2. Immunogenicity of the vaccine candidate in hamsters. (A) Hamsters were inoculated with 1 × 103 or 1 × 104 plaque-forming unit (PFU) of BK2102 intranasally, and the serum was collected 4 weeks after inoculation. Spike-specific IgG in the sera of BK2102-inoculated hamsters and mock- treated hamsters was detected by ELISA. Symbols depict data of individual hamsters (n=10), and bars correspond to the median value. The limit of dilution is indicated in the x-axis. (B) Neutralizing antibodies in the sera were induced in BK2102-inoculated hamsters. Neutralizing antibodies in the

Article Snippet: Biotinylated anti- Syrian hamster IgG1 Ab (Southern Biotech, Cat# 1940- 08, 1/100 dilution), IgG2/3 subclass Ab (Southern Biotech, Cat# 1935- 08, 1/200,000 dilution), and IgA Ab (Brookwood Biomedical, Cat# 3003a, 1/100 dilution) were used and detected with HRPlabeled streptavidin (Abcam, Cat# ab7403, 1/10,000 dilution).

Techniques: Immunopeptidomics, Enzyme-linked Immunosorbent Assay

Journal: STAR Protocols

Article Title: Protocol for preparation of primary alveolar epithelial type I cells from mouse lungs

doi: 10.1016/j.xpro.2024.103484

Figure Lengend Snippet: Characterization of purified AT1 cells (A) Immunofluorescence confocal microscope images of AT1 cells cultured for 5 days. Aquaporin5 (AQP5) is a marker of AT1 cells. Scale bar: 50 μm. (B) Quantification of AQP5-positive cells. (C) Dot plot of purified cells cultured for 5 days stained with anti-RAGE, AT1 cells were gated as the black polygon and singlets were gated as the diagonal. FSC, forward scatter; SSC, side scatter; FSC-H, forward scatter height; FSC-A, forward scatter area. (D) Dot plot of gated single AT1 cells with AF488 fluorescence intensity as X axis. (E) Dot plot of purified cells cultured for 5 days stained with anti-PDPN, AT1 cells were gated as the black polygon and singlets were gated as the diagonal. (F) Dot plot of gated single AT1 cells with CY3 fluorescence intensity as X axis. (G) Interleukin-6 concentration in the supernatants of AT1 cells incubated for 24 h with or without anti-RAGE antibody. (H) Viability of AT1 cells at different time points as day 5, 8, 12, and 14 (D5, D8, D12, D14). Data represent mean ± SD. ∗∗∗∗, p < 0.0001.

Article Snippet: Biotinylated Syrian hamster anti-mouse podoplanin (PDPN) monoclonal antibody (eBio8.1.1 (8.1.1)) (1:50 dilution) , Thermo Fisher Scientific , Cat# 14-5381-82; RRID: AB_1210505.

Techniques: Purification, Immunofluorescence, Microscopy, Cell Culture, Marker, Staining, Fluorescence, Concentration Assay, Incubation

Journal: STAR Protocols

Article Title: Protocol for preparation of primary alveolar epithelial type I cells from mouse lungs

doi: 10.1016/j.xpro.2024.103484

Figure Lengend Snippet:

Article Snippet: Biotinylated Syrian hamster anti-mouse podoplanin (PDPN) monoclonal antibody (eBio8.1.1 (8.1.1)) (1:50 dilution) , Thermo Fisher Scientific , Cat# 14-5381-82; RRID: AB_1210505.

Techniques: Recombinant, Modification, Electron Microscopy, Software, Sterility

Journal: PLoS ONE

Article Title: Nucleosomal Histone Proteins of L. donovani : A Combination of Recombinant H2A, H2B, H3 and H4 Proteins Were Highly Immunogenic and Offered Optimum Prophylactic Efficacy against Leishmania Challenge in Hamsters

doi: 10.1371/journal.pone.0097911

Figure Lengend Snippet: (a, b, c, d, e) and in patient sample (f, g, h, i, j). Serum samples were collected from different groups of hamsters at designated time points and assayed for specific IgG, IgG1, and IgG2 levels by ELISA. Each bar represents the pooled data (mean±S.D. value) of three replicates. Significance values indicate the difference between the cured group and normal group (***, p<0.001).

Article Snippet: Biotin-conjugated mouse anti-Armenian and Syrian hamster IgG, IgG1 and biotinylated anti-Syrian hamster IgG2 (BD Pharmingen) were added for 1 h at room temperature at 1/1000 dilutions and were further incubated with peroxidase-conjugated streptavidin at 1/1000 (BD Pharmingen) for 1 h. Finally, the substrate O-phenylenediamine dihydrochloride (Sigma) was added and the plate was read at 492 nm.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Nucleosomal Histone Proteins of L. donovani : A Combination of Recombinant H2A, H2B, H3 and H4 Proteins Were Highly Immunogenic and Offered Optimum Prophylactic Efficacy against Leishmania Challenge in Hamsters

doi: 10.1371/journal.pone.0097911

Figure Lengend Snippet: Serum samples were collected from different groups of hamsters at designated time points and assayed for specific IgG, IgG1, and IgG2 levels by ELISA. Significance values indicate the difference between the vaccinated group and infected group (***, p<0.001).

Article Snippet: Biotin-conjugated mouse anti-Armenian and Syrian hamster IgG, IgG1 and biotinylated anti-Syrian hamster IgG2 (BD Pharmingen) were added for 1 h at room temperature at 1/1000 dilutions and were further incubated with peroxidase-conjugated streptavidin at 1/1000 (BD Pharmingen) for 1 h. Finally, the substrate O-phenylenediamine dihydrochloride (Sigma) was added and the plate was read at 492 nm.

Techniques: Enzyme-linked Immunosorbent Assay, Infection